Foreword this edition of the difco manual, the eleventh published since 1927, has been extensively revised and rewritten. Eh infusionfree basal medium used with blood for enhanced betahemolytic reactions after overnight incubation and for cultivation of fastidious microorganisms. Yeast peptone dextrose agar for use with application. Food testing for staphylococcus aureus using bbl chromagar staph aureus.
Yeast extract is a source of the bcomplex vitamins. Bordetellosis shares many of the clinical and histopathological features of disease caused in mammals by bordetella pertussis and bordetella bronchiseptica. Ygc agar yeast extract glucose chloramphenicol agar filidf merck microbiology manual 12th edition 521 this culture medium complies with the recommendations of the international dairy federation internationaler milchwirtschaftsverband 1990, the international organization for standardization iso, the din norm 10186 for the examination. Mix 1 level teaspoon of dehydrated agar with 100 ml 38 cup of roomtemperature water. Bd is a leading medical technology company that advances human health in every corner of the world by improving medical research, diagnostics and the delivery of care. Bd difco microbiology difco culture media eugon agar difco 258910 500g. Xlt4 agar base used with xlt4 agar supplement in isolating. Dichloran rosebengal chloramphenicol drbc agar base. Pour 1012 ml of hot agar into each petri dish, just covering the. Solution is reddishpurple, very slightly to slightly opalescent.
Inoculum organism atcc cfu recovery haemophilus influenzae 10211 30300 good neisseria gonorrhoeae 43069 30300 good. Dotted lines on maps represent approximate border lines for which there may not yet be full. Bairdparker agar base bairdparker agar ey tellurite. Difco egg yolk enrichment 50% is a concentrated egg yolk emulsion recommended for use in a variety of media such as difco sfp agar base and difco mcclung toabe agar base for the isolation and identification of clostridium species on the basis of their lecithinase activity. Columbia blood agar base eh difco 279030 online ordering is required to receive these discount low prices. Distribute about 20 ml into petri dishes contained the test sample in advance, mix well, and incubate at. This agar was developed to address the special needs of the biotechnology industry for large scale. Bordetgengou agar was prepared as directed difco with 15% sheep blood added. Defibrinated animal blood supplies additional nutrients and enables the detection of hemolytic reactions, which aid in the identification of b. Gc agar base, with added blood or haemoglobin and other supplements is recommended for selective isolation and cultivation of gonococci. Journal of biological standardization 1975 3, 1129 the use of bordetella pertussis preserved m liquid nitrogen as a challenge suspension in the kendrick mouse protection test p. Suspend 46 g of the powder in 900 ml of purified water. Difco noble agar specifications and technical information.
Lactobacilli mrs agar is part of a large selection of dehydrated media. Plate count agar difco 247940 online ordering is required to. Enumeration of bacteria in water, wastewater, food and dairy. Dotted lines on maps represent approximate border lines for which there may not yet be. The use of bordetella pertussis preserved in liquid. Agar medium a medium 1 seed agar antibiotic penassay medium 2 medium 2 agar medium c medium 2 base agar antibiotic penassay medium 3 medium 3 broth medium a user quality control medium 3 broth. Dichloran rosebengal chloramphenicol drbc agar base is based on the formulation described by king et al.
Difco gc medium base, hemoglobin and supplement b or supplement vx prepare chocolate agar with gc medium base per label directions. Gc agar base has been formulated to include special peptone lp0072 which is a mixture of meat and plant enzymatic digests. Bd diagnostic systems regulatory regulatory documents. Bacto bordet gengou agar base is used with added blood for isolating. Bordetella avium virulence measured in vivo and in vitro europe. Bordetella avium causes an upperrespiratorytract disease called bordetellosis in birds. A agars bacto agar agar, grade a agar, granulated agar. Difco laboratories, division of becton dickinson and company sparks, maryland 21152 usa vi the difco manual foreword. Difco bbl manual manual of microbiological culture media. Iso ygc agar yeast extract glucose chloramphenicol agar. Selectively isolate neisseria species with thermo scientific oxoid gc agar base dehydrated. The purpose of the manual is to provide information about products used in microbiology. Bordet gengou agar base, with the addition of glycerol and.
Stainerscholte agar 18 was prepared with modifications for b. Composition ingredients gms litre peptone, special 15. Difco agar noble is a solidifying agent that is essentially free of impurities. Bordet gengou agar was prepared as directed difco with 15%. Tracheae from the shaminoculated birds had a welldefined ciliated border with a. Thermo scientific columbia blood agar base dehydrated.
It is used in electrophoretic and nutritional procedures and in preparing microbiological culture media when increased purity is required. Building on this knowledge base, difco laboratories continued to develop more. Manual of microbiological culture media and a great selection of related books, art and collectibles available now at. Bordet gengou agar base, with the addition of glycerol and sterile blood, is. The peptones and beef extract provide amino acids and other complex nitrogenous nutrients. Also, manual gauging is used for shipping tanks such as barges, tankers and trailers. This chapter describes menterococcus me agar as an improved version of the original medium of slanetz. Blood agar base media are specified in standard methods for food testing. Rosebengal in the medium controls the size and height of mold colonies preventing overgrowth and making. Tryptone glucose yeast agar are used for obtaining microbial plate counts from milk and dairy products, foods, water and other materials of sanitary importance.
Bring to a boil while stirring to ensure the agar is completely dissolved. The agar id310 portable emulsion profiler is used for these applications where a portable device is more practical than a permanent system. The cumulative effect of these modifications is to further inhibit bacterial growth, inhibit spreading moulds such as rhizopus and mucor and make the medium capable of supporting the growth of those species that cannot be isolated on rosebengal chloramphenicol agar or acidified potato dextrose agar 1. There are no alternate images available for this product. Laboratory manual for the diagnosis of whooping cough caused by. Dehydrated culture media and ingredients ordering table certain formulations are designated by a ii in the medium name.
Brookes\ cultures of bordetella pertussis, strain 18323, grown in liquid defined medium were frozen in the presence of 10% vv glycerol in liquid nitrogen. The presence of starch in the medium ensures that toxic metabolites produced by nei. Bd leads in health care safety including medication safety, health care worker safety. Mcp agar base is prepared as per the formula of armon and payment 2. Thermo scientific columbia blood agar base dehydrated 10188142 print thermo scientific columbia blood agar base dehydrated cultivate fastidious microorganisms with clear hemolytic reactions and good colonial differentiation with this generalpurpose blood agar base. Gonococcal gc medium base is part of a large selection of dehydrated media. Light beige to light pinkish beige, freeflowing, homogeneous. Makes 100 ml of nutrient agar solution enough to cover 79 standard 90100 mm petri dishes. It is also recommended by the directive of the council of the european union 9883ec 3 for isolation and enumeration of clostridium perfringens from water sample using membrane filtration technique. Agar granulated may be used for general bacteriological purposes where clarity is not a strict requirement.
Difco columbia blood agar base eh 279030 global scientific. Bordetgengou agar is a type of agar plate optimized to isolate bordetella, containing blood, potato extract, and glycerol, with an antibiotic such as cephalexin or. Bordetella avium virulence measured in vivo and in vitro. These formulations are improved to provide more consistent, superior test results than earlier versions. For various additives in isolating and cultivating neisseria gonorrhoeae and other fastidious microorganisms. Border gengou agar base, with the addition of glycerol and sterile blood, is.
Atcc 8467 identity specifications difco bordet gengou agar base dehydrated. The medium relies upon the selective inhibitory properties of sodium azide and the incorporation of tetrazolium that most organisms growing on this medium will reduce to some extent under the conditions provided. Bordet gengou blood agar contains potato infusion and glycerol to supply the nutrients necessary to support the growth of b. Bordetella avium virulence measured in vivo and in vitro infection. Supplementing casman agar base with blood supplies the growth factors required by h. After rehydration, the strains were cultured on blood agar base plates bab no. Intended use hardy diagnostics pc pseudomonas cepacia agar is recommended for the selective isolation of burkholderia pseudomonas cepacia from clinical and environmental materials. Macconkey agar base is used with added carbohydrate in. Bg sulfa agar difco 271710 buy online through voigt global distribution, your dealer distributor for all bbl difco bd microbiology products. Noble agar difco media distributor pharmaceuticals. Development of a selective culture medium for primary isolation. Bl agar for isolation of anaerobes and differentiation of bifidobacterium formula components meat extract proteose peptone peptone soya peptone yeast extract liver extract dextrose soluble starch monopotassium phosphate potassium dlhydrogen phosphate magnesium sulfate. Pdf bordetella avium virulence measured in vivo and in vitro. Agar granulated is qualified to grow recombinant strains of escherichia coli hb101 and saccharomyces cerevisiae.